Production of Lipase by Immobilized Bacillus thuringiensis and Their Biodegradation Potential on Premium Motor Spirit
Keywords:
Bacillus thuringiensis, Immobilization, Biodegradation, Lipase, PMS, GC-MS, Bushnell-Hass Mineral Salt mediumAbstract
The synthesis of lipase by immobilized Bacillus thuringiensis was described in this work. Bacteria isolates were tested for oil degradation on Bushnell-Hass Mineral Salt medium with 1% v/v PMS. Bacillus thuringiensis was identified as the potent isolate utilizing 16S rRNA. The isolates were immobilized in gelatin matrix and grown in a submerged medium for lipase synthesis. The crude lipase extracted was used for degradation of PMS. Lipase from immobilized Bacillus thuringiensis achieved optimal PMS degradation of 50.9% in 20 days at pH 7 and 35°C. The chemicals decomposed within 20 days, according to GC-MS analyses. As a result, our study demonstrated that using immobilized bacterial lipase to degrade PMS is a simple and effective method.
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